Anti-AP2 alpha抗体- ChIP Grade (ab52222)

概述

  • 产品名称Anti-AP2 alpha抗体- ChIP Grade
    参阅全部 AP2 alpha 一抗
  • 描述
    兔多克隆抗体to AP2 alpha - ChIP Grade
  • 特异性ab52222 detects endogenous levels of total AP2 alpha protein.
  • 经测试应用适用于: ChIP, ICC/IF, EMSA, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide derived from human AP2 alpha.

  • 阳性对照
    • Extracts from COLO205 cells

性能

应用

Our Abpromise guarantee covers the use of ab52222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
ICC/IF 1/1000. (see Abreview)
EMSA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml.
EMSA Use at an assay dependent concentration. PubMed: 20671194

靶标

  • 功能Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle.
  • 疾病相关Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies.
  • 序列相似性Belongs to the AP-2 family.
  • 结构域The WW-binding motif mediates interaction with WWOX.
  • 翻译后修饰Sumoylated on Lys-10; which inhibits transcriptional activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating enhancer binding protein 2 alpha antibody
    • Activating enhancer-binding protein 2-alpha antibody
    • Activator protein 2 antibody
    • AP 2 transcription factor antibody
    • AP 2alpha antibody
    • AP-2 antibody
    • AP-2 transcription factor antibody
    • AP2 antibody
    • AP2 Transcription Factor antibody
    • AP2-alpha antibody
    • AP2A_HUMAN antibody
    • AP2TF antibody
    • BOFS antibody
    • FLJ51761 antibody
    • TFAP 2 antibody
    • TFAP 2A antibody
    • TFAP2 antibody
    • TFAP2A antibody
    • Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody
    • Transcription factor AP-2-alpha antibody
    • Transcription factor AP2 alpha antibody
    see all

Anti-AP2 alpha antibody - ChIP Grade 图像

  • All lanes : Anti-AP2 alpha antibody - ChIP Grade (ab52222) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells,
    Lane 2 : extracts from COLO205 cells, with immunizing peptide


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded murine embryonic tissue sections, labelling AP2 Alpha with ab52222 at a dilution of 1/3000 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary was a goat anti-rabbit polyclonal biotin conjugate at 1/300. In this day18 embryo, nuclear positivity was also detected in spinal cord, ganglia, kidney tubules and the keratinised portion of the stomach.

    See Abreview

  • Antibody anti-AP-2 alpha (ab52222) was used in an Electrophoretic Mobility Shift Assay (EMSA) to supershift the protein-DNA complex. Radiolabelled, double-stranded DNA oligonucleotides (10.000 cpm per lane) harbouring a binding site for AP-2 alpha were incubated with each 2 µg of nuclear extract (NE) from HeLa and Caski cells, respectively. Samples were incubated for 30 minutes at room temperature to allow the formation of protein-DNA complexes. 2 µg of anti-AP-2 alpha antibody were added to the samples (as indicated) and incubated for further 60 minutes at 4°C. Samples were separated in a 5.5% PAGE for 30 minutes at 280 V and further 75 minutes at 350 V. The Gel was dried under vacuum and for autoradiography a X-ray film was exposed with an intensifying screen for 2 days at -80°C. Specific protein-DNA complexes were quantitatively supershifted with antibody anti-AP-2 alpha (ab52222), verifying the binding of AP-2 alpha to the DNA oligonucleotide. Rating: ***** This image was
  • IHC image of AP2 alpha staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52222, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-AP2 alpha antibody - ChIP Grade (ab52222)参考文献

This product has been referenced in:

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS7)
Permeabilization Yes - 0.2%Triton
Specification COS7
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embryo skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Embryo skin
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Embryonic spinal cord)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Embryonic spinal cord
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 21 2015

Thank you for contacting us.

The exact immunogen sequence is not available to us as the originating laboratory considers it proprietary, but we at least know that it is within the final 30 amino acids of the C terminus of the human protein, ...

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Thank you for your inquiry.

I am happy to confirm that ab500 (ChIP Kit) is suitable for this antibody since it can be used with rabbit antibodies.

http://www.abcam.com/index.html?datasheet=500 (or use the following: http://www.abcam...

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Thank you for contacting us.

Reactivity with chicken AP2-2 alpha is predicted based on 100% conservation of the immunogen in the chicken protein NP_990425.1.

However, we do not have data confirming this, and reactivity with chicke...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human cervical cell lines)
Specification human cervical cell lines
Fixative Paraformaldehyde
Permeabilization Yes - triton
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (Cervical cancer cell lines)
Specification Cervical cancer cell lines
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Semiquantitative PCR
Positive control Tumor cell lines Hela
Negative control Human primary keratinocytes
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Abcam user community

Verified customer

提交于 Jan 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human keratinocytes)
Specification human keratinocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human keratinocytes)
Loading amount 50 µg
Specification Human keratinocytes
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

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提交于 Nov 15 2010

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