The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
序列相似性Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.
翻译后修饰Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.
细胞定位Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.