概述

  • 产品名称
  • 描述
    兔多克隆抗体to AP1AR
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence GNCCWTQCFG LLRKEAGRLQ RVGGGGGSKY FRTCSRGEHL TIEFENLVES DEGESPGSSH RPLTEEEIVD LRERHYDSIA E, corresponding to N terminal amino acids 2-82 of Human AP1AR (UniProt ID: Q63HQ0).

  • 阳性对照
    • IHC-P: Human oral mucosa ICC/IF: A431 cells

性能

应用

Our Abpromise guarantee covers the use of ab122296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.

靶标

  • 功能
    Necessary for AP-1 dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/Gamma1-adaptin, one of the subunits of the AP-1 adapter complex. The direct interaction with AP1G1/Gamma1-adaptin attenuates the release of the AP-1 complex from membranes.
  • 细胞定位
    Golgi apparatus > trans-Golgi network. Late endosome. Early endosome. Localizes to the juxta-nuclear Golgi region and to tubular structures throughout the cytoplasm, which are highly mobile and cycle between the juxta-nuclear area and the cell periphery.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2c18 antibody
    • Adapter-related protein complex 1-associated regulatory protein antibody
    • AP-1 complex-associated regulatory protein antibody
    • AP1AR antibody
    • AP1AR_HUMAN antibody
    • C4orf16 antibody
    • Chromosome 4 open reading frame 16 antibody
    • Gamma-1-adaptin brefeldin A resistance protein antibody
    • Gamma-BAR antibody
    • Gamma1-adaptin brefeldin A resistance protein, gamma-BAR antibody
    • GBAR antibody
    • PRO0971 antibody
    see all

Anti-AP1AR antibody 图像

  • ab122296, at a 1/200 dilution, staining AP1AR in paraffin embedded Human oral mucosa by Immunohistochemistry.
  • ab122296, at 4 µg/ml, staining AP1AR in A431 cells by Immunofluorescence.

Anti-AP1AR antibody (ab122296)参考文献

This product has been referenced in:
  • Hirst J  et al. Contributions of epsinR and gadkin to clathrin-mediated intracellular trafficking. Mol Biol Cell 26:3085-103 (2015). Read more (PubMed: 26179914) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 18°C
Sample
Mouse Cell (immortalized MEFs)
Specification
immortalized MEFs
Permeabilization
Yes - 0.3% Triton X-100 for 5min
Fixative
Methanol
Username

Dr. Hannah Schachtner

Verified customer

提交于 Oct 07 2013

Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (immortalized MEFs)
Specification
immortalized MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

提交于 Oct 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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