Anti-AP180抗体(ab33898)

概述

  • 产品名称Anti-AP180抗体
  • 描述
    兔多克隆抗体to AP180
  • 经测试应用适用于: ICC/IF, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Rat AP180.

    (Peptide available as ab33897.)

  • 阳性对照
    • ab33898 gave a positive signal in Rat Brain, Mouse Brain, and Human Brain Tissue Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab33898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 168 kDa (predicted molecular weight: 93 kDa).
IHC-FoFr 1/1000.

靶标

  • 相关性AP180 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats. This protein is also known as pp155, NP185, F1-20, and AP-3. During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.
  • 细胞定位Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
  • 数据库链接
  • 别名
    • 91 kDa synaptosomal associated protein antibody
    • AP 180 antibody
    • AP3 antibody
    • Assembly Protein 180 kDa antibody
    • Assembly Protein AP180 antibody
    • CALM antibody
    • Clathrin coat assembly protein antibody
    • Clathrin coat assembly protein AP180 antibody
    • Clathrin coat associated protein AP180 antibody
    • DKFZp781O051 antibody
    • F120 antibody
    • KIAA0656 antibody
    • NP185 antibody
    • pp155 antibody
    • SNAP 91 antibody
    • SNAP91 antibody
    • Synaptosomal associated protein 91 kD homolog antibody
    • Synaptosomal associated protein 91 kDa homolog antibody
    see all

Anti-AP180 antibody 图像

  • All lanes : Anti-AP180 antibody (ab33898) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Brain (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 168 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    AP180 is a brain specific protein that contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. AP180 has also been shown to run in western blot at approximately 180 kDa in numerous publications, e.g. Am J Physiol Cell Physiol 285: C995-C1008, 2003 (PMID: 14532018).

  • ICC/IF image of ab33898 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33898, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • Immunostaining of Mouse Striatum suing Ab33898. The tissues are from perfused fixed animals perfused with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat and stained by direct fluorescence.

    See Abreview

Anti-AP180 antibody (ab33898)参考文献

ab33898 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections - Striatum)
Specification Brain sections - Striatum
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
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Dr. Sophie Pezet

Verified customer

提交于 Mar 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"