The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/500 - 1/5000. depending on tissue and
IHC-P: 1/500 - 1/5000. depending on tissue and
WB: 1/2000 - 1/10000. depending on detection method. Predicted molecular weight: 100 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Subunit of clathrin associated adaptor protein complex 1 that plays a role in protein sorting in the late Golgi/trans Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Adaptor protein complex 1 (AP1) is an heterotetramer composed of two large adaptins (gamma type subunit AP1G1 and beta type subunit AP1B1), a medium adaptin (mu type subunit AP1M1 or AP1M2) and a small adaptin (sigma type subunit AP1S1 or AP1S2 or AP1S3).
Poulsen ET et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci10:59 (2017).
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