Anti-Antithrombin III抗体[BDI205] (ab20933)

概述

  • 产品名称Anti-Antithrombin III抗体[BDI205]
    参阅全部 Antithrombin III 一抗
  • 描述
    小鼠单克隆抗体[BDI205] to Antithrombin III
  • 经测试应用适用于: WB, Blocking, ELISA, Dot blot, Purification, ICC/IF, IHC-P, IPmore details
  • 种属反应性
    与反应: Human, Baboon
    不与反应: Rabbit, Cow, Dog
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照
    • This antibody gave a positive signal in ICC/IF in HepG2 cell line. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 150mM Sodium chloride, 10mM Sodium phosphate. pH 7.4
  • Concentration information loading...
  • 纯化说明Purified from ascites by Gel filtration and anion exchange.
  • 克隆单克隆
  • 克隆编号BDI205
  • 骨髓瘤NS1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
Blocking Use at an assay dependent concentration.
ELISA Use a concentration of 1 - 5 µg/ml.
Dot blot Use a concentration of 1 - 5 µg/ml.
Purification Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

靶标

  • 功能Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 组织特异性Found in plasma.
  • 疾病相关Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • 序列相似性Belongs to the serpin family.
  • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

Anti-Antithrombin III antibody [BDI205] 图像

  • IHC image of Antithrombin III staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20933, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab20933 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20933, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Antithrombin III antibody [BDI205] (ab20933)参考文献

This product has been referenced in:
  • Gore S  et al. Heparin surfaces: Impact of immobilization chemistry on hemocompatibility and protein adsorption. J Biomed Mater Res B Appl Biomater 102:1817-24 (2014). WB . Read more (PubMed: 24711209) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. The immunogen used to develop ab20933, MAb to Antithrombin III, was purified full-length Human Antithrombin III. The epitope has not been mapped for this antibody, therefore we cannot guarantee that it will recognize you...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (CHO cells transfected with human Antithrombin III)
Total protein in input 150000 cells
Specification CHO cells transfected with human Antithrombin III
Immuno-precipitation step Protein G
Username

Ning Wang

Verified customer

提交于 Oct 31 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"