Anti-Antithrombin III抗体(ab8776)


  • 产品名称Anti-Antithrombin III抗体
    参阅全部 Antithrombin III 一抗
  • 描述
    羊多克隆抗体to Antithrombin III
  • 特异性This product gives a single arc when tested by IEP and 2D-IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs human plasma and a known anti-human antithrombin III.
  • 经测试应用适用于: Double Immunodiffusion, RID, Immunoelectrophoresismore details
  • 种属反应性
    与反应: Mouse, Rat, Horse, Cat, Dog, Human
    不与反应: Rabbit, Chicken, Guinea pig, Cow, Pig
  • 免疫原

    Human antithrombin III purified from plasma, shown to be homogeneous by SDS-PAGE.

  • 常规说明The concentration varies from batch to batch (10-20 mg/mL).


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Antiserum is prepared by immunisation of sheep with Antithrombin III and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2µm filtered.
  • 克隆多克隆
  • 同种型IgG
  • 轻链类型unknown
  • 研究领域


Our Abpromise guarantee covers the use of ab8776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Double Immunodiffusion
Rocket Immunoelectrophoresis
  • 应用说明RID and Rocket IEP: 6µL antiserum/cm² gel vs 5µL neat – 1/10 dilution human plasma
    Double diffusion: 10µL antiserum vs 5µL plasma
    IEP: 100µL antiserum vs 5µL plasma
    The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
    Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded.
  • 靶标

    • 功能Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
    • 组织特异性Found in plasma.
    • 疾病相关Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
    • 序列相似性Belongs to the serpin family.
    • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
    • 细胞定位Secreted > extracellular space.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ANT3_HUMAN antibody
      • Antithrombin antibody
      • Antithrombin III antibody
      • Antithrombin-III antibody
      • AntithrombinIII antibody
      • AT 3 antibody
      • AT III antibody
      • AT3 antibody
      • AT3D antibody
      • ATIII antibody
      • Heparin cofactor I antibody
      • MGC22579 antibody
      • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
      • Serine cysteine proteinase inhibitor clade C member 1 antibody
      • Serine proteinase inhibitor clade C member 1 antibody
      • Serpin C1 antibody
      • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
      • SERPINC1 antibody
      • THPH7 antibody
      see all

    Anti-Antithrombin III antibody (ab8776)参考文献

    ab8776 has not yet been referenced specifically in any publications.

    Product Wall

    I have received the following protocol for ab13335 in direct ELISA (not sandwich as sandwich ELISA has not been tested with ab13335) which I hope will help you. 1. Coating 1-5 ug/ml per well of Anti-thrombin III on High binding plates. Incubation fo...

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    Thank you for your email. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get o...

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    Thank you for your enquiry. We are sorry to inform you that there is no protein concentration for polyclonal antibodies that are not affinity purified. If you have any more questions, please contact us again.