Antigen Retrieval缓冲液(100X Tris-EDTA缓冲液, pH 9.0) (ab93684)

概述

  • 产品名称Antigen Retrieval缓冲液(100X Tris-EDTA缓冲液, pH 9.0)
    参阅全部 Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) 试剂
  • 描述
    Antigen Retrieval缓冲液(100X Tris-EDTA缓冲液, pH 9.0)
  • 经测试应用适用于: IHC-Pmore details
  • 常规说明PROTOCOL:
    1. Place slides in a Coplin jar containing 1x Antigen Retrieval Buffer; cover with a vented plastic wrap and place the jar in microwave and set high power to boil and set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
    2. Wash sections with hot tap water for 1 minute.
    3. Wash sections in buffer for 2x3 minutes.
    4. Block endogenous peroxidase as usual.
    5. Wash sections in buffer for 2x3 minutes.
    6. Block non-specific sites with a Blocking reagent as usual.
    7. Place optimally diluted primary antibody on the sections (incubation time and temperature for a given set of experimental conditions should be determined by the investigator).
    8. Wash sections in buffer for 2x3 minutes
    9. Rest of the procedure is same as routinely performed in your laboratory.

性能

  • 相关性Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH=9.0) pretreatment.

应用

Our Abpromise guarantee covers the use of ab93684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.

Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)参考文献

This product has been referenced in:
  • Li Y  et al. Perinatal nicotine exposure increases vulnerability of hypoxic-ischemic brain injury in neonatal rats: role of angiotensin II receptors. Stroke 43:2483-90 (2012). Read more (PubMed: 22738920) »

See 1 Publication for this product

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Thank you for calling Abcam earlier today.

Please find the recommended protocol to use with ab93684 attached.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"