Antigen Retrieval缓冲液(100X EDTA缓冲液,pH 8.0) (ab93680)
概述
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产品名称
Antigen Retrieval缓冲液(100X EDTA缓冲液,pH 8.0) -
经测试应用
适用于: IHC-Pmore details -
常规说明
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require EDTA buffer (pH=8.0) pretreatment.
This product contains detergent for emulsification of the paraffin.
1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.
PROTOCOL:
- Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
- Wash sections with hot tap water for 1 minute.
- Wash sections in buffer for 2x3 minutes.
- Continue with IHC protocol as normal.
Other kits and reagents for IHC
Other antigen retrieval buffers include:
Citrate buffer pH 6.0 ab93678, Tris-EDTA buffer pH 9.0 ab93684, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
性能
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形式
Liquid -
存放说明
Store at room temperature. -
存储溶液
pH: 8
Constituents: 1.5% 2-butoxyethanol, 5% Sodium EDTA
Buffer 100x Concentrated with detergent. -
Concentration information loading...
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93680于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent dilution.
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说明 |
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IHC-P
Use at an assay dependent dilution. |
图片
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Prolonged survival of Lgr5/Apc and MYC-ON mice depends on the T cells.g Immunohistochemical staining of GFP in the indicated genotypes (n = 5 mice per group).
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Ophiocordyceps formosana upregulated adiponectin expressions in plasma and adipose tissuesb) Immunohistochemical staining for adiponectin in adipose tissues.
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ab93680 Antigen Retrieval Buffer 100X EDTA Buffer, pH 8
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab93680 被引用在 4 文献中.
- Wang Y et al. Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. Elife 9:N/A (2020). PubMed: 32479261
- Wang G et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun 11:220 (2020). PubMed: 31924786
- Huang CW et al. Ophiocordyceps formosana improves hyperglycemia and depression-like behavior in an STZ-induced diabetic mouse model. BMC Complement Altern Med 16:310 (2016). IHC-P . PubMed: 27553852
- Zhu Y et al. Elevated expression of AKT2 correlates with disease severity and poor prognosis in human osteosarcoma. Mol Med Rep 10:737-42 (2014). PubMed: 24919955