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Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require citrate buffer (pH=6.0) pretreatment.
This product contains citrate, pH 6.0 and detergent for emulsification of the paraffin.
1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.
1. Place slides in a Coplin jar containing 1x Target Retrieval Buffer; cover with a vented plastic wrap and place the jar in microwave and set high power to boil and set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20 min.
2. Wash sections with hot tap water for 1 min.
3. Wash sections in buffer for 2 x 3 minutes.
4. Block endogenous peroxidase as usual.
5. Wash sections in buffer for 2 x 3 minutes.
6. Block non-specific sites with a Blocking reagent as usual.
7. Place optimally diluted primary antibody on the sections (incubation time and temperature for a given set of experimental conditions should be determined by the investigator).
8. Wash sections in buffer for 2 x 3 minutes.
9. Rest of the procedure is same as routinely performed in your laboratory.
Our Abpromise guarantee covers the use of ab93678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"