Anti-Anterior Gradient 2抗体(ab22208)

概述

  • 产品名称Anti-Anterior Gradient 2抗体
    参阅全部 Anterior Gradient 2 一抗
  • 描述
    兔多克隆抗体to Anterior Gradient 2
  • 特异性ab22208 reacts with Anterior Gradient 2 (AGR2) Homolog.
  • 经测试应用适用于: ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide:

    TQTYEEALYK SKTSNKPL

    , corresponding to internal sequence amino acids 55-72 of Human Anterior Gradient 2.

  • 阳性对照
    • Normal colon tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab22208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/100 - 1/1000.
IHC-P 1/100.
ICC/IF 1/200. PubMed: 19359471

靶标

  • 功能Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
  • 组织特异性Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
  • 序列相似性Belongs to the AGR family.
  • 细胞定位Secreted. Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AG 2 antibody
    • AG 2 protein antibody
    • AG-2 antibody
    • AG2 antibody
    • AG2 protein antibody
    • AGR 2 antibody
    • AGR2 antibody
    • AGR2_HUMAN antibody
    • Anterior gradient 2 homolog (Xenopus laevis) antibody
    • anterior gradient 2 homolog antibody
    • Anterior gradient 2, Xenopus, homolog of antibody
    • Anterior gradient homolog 2 antibody
    • Anterior gradient protein 2 homolog antibody
    • GOB 4 antibody
    • GOB4 antibody
    • HAG 2 antibody
    • hAG-2 antibody
    • hAG2 antibody
    • HAG2/R antibody
    • HPC 8 antibody
    • hPC8 antibody
    • PDIA17 antibody
    • Protein disulfide isomerase family A member 17 antibody
    • secreted cement gland homolog antibody
    • Secreted cement gland protein XAG 2 homolog antibody
    • Secreted cement gland protein XAG-2 homolog antibody
    • Secreted cement gland protein XAG2 homolog antibody
    • XAG 2 antibody
    • XAG2 antibody
    see all

Anti-Anterior Gradient 2 antibody 图像

  • ab22208 staining Anterior Gradient 2 in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An  Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical staining with ab22208 in normal colon tissue at 1:100 dilution.
  • ab22208 staining Anterior Gradient 2 in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-Anterior Gradient 2 antibody (ab22208)参考文献

This product has been referenced in:
  • Dumartin L  et al. AGR2 is a novel surface antigen that promotes the dissemination of pancreatic cancer cells through regulation of cathepsins B and D. Cancer Res 71:7091-102 (2011). Read more (PubMed: 21948970) »
  • Park SW  et al. The protein disulfide isomerase AGR2 is essential for production of intestinal mucus. Proc Natl Acad Sci U S A 106:6950-5 (2009). ICC/IF, IHC-P ; Mouse . Read more (PubMed: 19359471) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Mrs. ice cool

Verified customer

提交于 Mar 19 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS1 cell line)
Specification COS1 cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 11 2013

Thank you for your rapid reply. I understand that you are concerned about the quality of ab30927 and take your comments very seriously. Please be assured that should we suspect that the quality of one of our products is not of the highest standards we ...

Read More

I'm sorry to hear you are having a problem with ab22208. As noted on the datasheet, this antibody has not been tested for Western blotting, only ELISA and IHC. However, the following protocol modifications may improve your results: 1. Fully denature...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"