概述

  • 产品名称
    Anti-ANP抗体- N-terminal
    参阅全部 ANP 一抗
  • 描述
    兔多克隆抗体to ANP - N-terminal
  • 经测试应用
    适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to Human ANP aa 30-56 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 4878

  • 阳性对照
    • Mouse heart tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human heart muscle.

性能

应用

Our Abpromise guarantee covers the use of ab91250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.
ELISA 1/1000.

靶标

  • 功能
    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • 疾病相关
    Atrial standstill 2
    Atrial fibrillation, familial, 6
  • 序列相似性
    Belongs to the natriuretic peptide family.
  • 翻译后修饰
    Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANF antibody
    • ANF_HUMAN antibody
    • ANP antibody
    • ATFB6 antibody
    • Atrial natriuretic factor antibody
    • Atrial natriuretic peptide antibody
    • Atriopeptin antibody
    • Cardiodilatin antibody
    • Cardiodilatin-related peptide antibody
    • Cardionatrin antibody
    • CDD ANF antibody
    • CDD antibody
    • CDD-ANF antibody
    • CDP antibody
    • Natriuretic peptide A antibody
    • Natriuretic peptide precursor A antibody
    • NPPA antibody
    • PND antibody
    • Prepronatriodilatin antibody
    see all

Anti-ANP antibody - N-terminal 图像

  • Anti-ANP antibody - N-terminal (ab91250) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 17 kDa
  • IHC image of ANP staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91250, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ANP antibody - N-terminal (ab91250)参考文献

This product has been referenced in:
  • Medford HM  et al. Consuming a Western diet for two weeks suppresses fetal genes in mouse hearts. Am J Physiol Regul Integr Comp Physiol 306:R519-26 (2014). Read more (PubMed: 24523346) »
  • Medford HM  et al. Immediate effects of a single exercise bout on protein O-GlcNAcylation and chromatin regulation of cardiac hypertrophy. Am J Physiol Heart Circ Physiol 305:H114-23 (2013). WB . Read more (PubMed: 23624624) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Dog Tissue lysate - whole (atrium and ventricle)
Loading amount
75 µg
Specification
atrium and ventricle
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Dec 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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