概述

  • 产品名称Anti-ANP抗体
    参阅全部 ANP 一抗
  • 描述
    兔多克隆抗体to ANP
  • 特异性Reacts with Atrial Natriuretic Polypeptide (human) 100% and Atrial Natriuretic Polypeptide (rat) ~ 60% Does not cross react with ACTH, Calcitonin (salmon), PTH (human 1-84) or Vasoactive Intestinal Peptide
  • 经测试应用适用于: ICC/IF, IPmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Human atrial natriuretic polypeptide covalently attached onto a carrier protein.

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145

性能

应用

Our Abpromise guarantee covers the use of ab40791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
IP 1/1000 - 1/10000. Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking.

靶标

  • 功能Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • 疾病相关Atrial standstill 2
    Atrial fibrillation, familial, 6
  • 序列相似性Belongs to the natriuretic peptide family.
  • 翻译后修饰Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANF antibody
    • ANF_HUMAN antibody
    • ANP antibody
    • ATFB6 antibody
    • Atrial natriuretic factor antibody
    • Atrial natriuretic peptide antibody
    • Atriopeptin antibody
    • Cardiodilatin antibody
    • Cardiodilatin-related peptide antibody
    • Cardionatrin antibody
    • CDD ANF antibody
    • CDD antibody
    • CDD-ANF antibody
    • CDP antibody
    • Natriuretic peptide A antibody
    • Natriuretic peptide precursor A antibody
    • NPPA antibody
    • PND antibody
    • Prepronatriodilatin antibody
    see all

Anti-ANP antibody 图像

  • ICC/IF image of ab40791 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40791 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ANP antibody (ab40791)参考文献

ab40791 has not yet been referenced specifically in any publications.

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