Anti-Anoctamin 3抗体(ab82781)

概述

  • 产品名称Anti-Anoctamin 3抗体
  • 描述
    兔多克隆抗体to Anoctamin 3
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 828-877 (AFVIAITSDY IPRFVYEYKY GPCANHVEPS ENCLKGYVNN SLSFFDLSEL) of human Anoctamin 3 (NP_113606).

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 115 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Anoctamin 3 antibody 图像

  • Anti-Anoctamin 3 antibody (ab82781) at 1 µg/ml (5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 115 kDa
    Observed band size : 115 kDa
  • Immunohistochemical analysis of porcine retina tissue, staining Anoctamin 3 with ab82781.

    Tissue was fixed with paraformaldehyde and blocked with blocking reagent for 30 minutes at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/50 in diluent) for 24 hours at 4°C. A biotinylated anti-rabbit IgG was used as the secondary antibody.

    See Abreview

Anti-Anoctamin 3 antibody (ab82781)参考文献

ab82781 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (retina)
Specification retina
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step (agent) for 30 minute(s) · Concentration: 0% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 17 2012

DISCOUNT CODE: 100%ABR-0000

Ablaufdatum: 16. November 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörperab82781 inSchwein zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"