Anti-Anoctamin 3抗体(ab82781)

概述

  • 产品名称
    Anti-Anoctamin 3抗体
  • 描述
    兔多克隆抗体to Anoctamin 3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 828-877 (AFVIAITSDY IPRFVYEYKY GPCANHVEPS ENCLKGYVNN SLSFFDLSEL) of human Anoctamin 3 (NP_113606).

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 115 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-Anoctamin 3 antibody (ab82781) at 1 µg/ml (5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 115 kDa
    Observed band size: 115 kDa



    Gel concentration: 6-18%
  • Immunohistochemical analysis of porcine retina tissue, staining Anoctamin 3 with ab82781.

    Tissue was fixed with paraformaldehyde and blocked with blocking reagent for 30 minutes at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/50 in diluent) for 24 hours at 4°C. A biotinylated anti-rabbit IgG was used as the secondary antibody.

    See Abreview

文献

ab82781 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (retina)
Specification
retina
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 0% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 17 2012

DISCOUNT CODE: 100%ABR-0000

Ablaufdatum: 16. November 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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