Annexin VII overexpression 293T裂解液(全细胞) (ab94175)

概述

  • 产品名称Annexin VII overexpression 293T裂解液(全细胞)
  • 描述
    Annexin VII overexpression裂解液
  • 常规说明ab94175 is a 293T cell transfected lysate in which Human Annexin VII has been transiently over-expressed using a pCMV-Annexin VII plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Tissue specificity: Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.

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应用

Our Abpromise guarantee covers the use of ab94175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Annexin VII overexpression 293T lysate (whole cell) 图像

  • ab94175 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Annexin VII antibody (ab88539) at 1/500 dilution

    Lane 1 : Annexin VII overexpression 293T lysate (whole cell) (ab94175)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Annexin VII overexpression 293T lysate (whole cell) (ab94175)参考文献

ab94175 has not yet been referenced specifically in any publications.

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