Anti-Annexin VII抗体[203-217/6] (ab49838)

概述

  • 产品名称Anti-Annexin VII抗体[203-217/6]
    参阅全部 Annexin VII 一抗
  • 描述
    小鼠单克隆抗体[203-217/6] to Annexin VII
  • 经测试应用适用于: IHC-Fr, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human
  • 免疫原

    Recombinant full length protein (Mouse)

  • 阳性对照
    • Whole cell extract of culture mouse connective tissue (A9) cells.

性能

应用

Our Abpromise guarantee covers the use of ab49838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
ICC
WB
  • 应用说明ICC: Use at an assay dependent dilution with methanol or 1% paraformaldehyde/ 0.1% glutaraldehyde.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 47 or 51 kDa depending on the tissue/cell used (predicted molecular weight: 53 kDa). A degradation product of approximately 30 kDa may also be detected.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Annexin VII antibody [203-217/6] 图像

    • Anti-Annexin VII antibody [203-217/6] (ab49838) at 1/1000 dilution + whole cell lysate prepared from human oesophageal squamous cell carcinoma PJ41 cells at 20 µg

      Secondary
      Goat anti-mouse IgG conjugated to HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    Anti-Annexin VII antibody [203-217/6] (ab49838)参考文献

    ab49838 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Oral)
    Loading amount 20 µg
    Specification Oral
    Gel Running Conditions Reduced Non-Denaturing (Native)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    提交于 Jan 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"