Anti-Annexin VI抗体(ab52221)

概述

  • 产品名称Anti-Annexin VI抗体
    参阅全部 Annexin VI 一抗
  • 描述
    兔多克隆抗体to Annexin VI
  • 特异性ab52221 detects endogenous levels of total Annexin A6 protein
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from human Annexin A6.

  • 阳性对照
    • Extracts from 293 cells

性能

应用

Our Abpromise guarantee covers the use of ab52221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
  • 序列相似性Belongs to the annexin family.
    Contains 8 annexin repeats.
  • 结构域A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰Phosphorylated in response to growth factor stimulation.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 67 kDa calelectrin antibody
    • Annexin A6 antibody
    • Annexin VI antibody
    • Annexin VI p68 antibody
    • Annexin-6 antibody
    • AnnexinA6 antibody
    • AnnexinVI antibody
    • ANX 6 antibody
    • ANX A6 antibody
    • ANX6 antibody
    • ANXA 6 antibody
    • ANXA6 antibody
    • ANXA6_HUMAN antibody
    • Calcium binding protein p68 antibody
    • Calelectrin antibody
    • Calphobindin II antibody
    • Calphobindin-II antibody
    • CalphobindinII antibody
    • CBP 68 antibody
    • CBP68 antibody
    • Chromobindin 20 antibody
    • Chromobindin-20 antibody
    • Chromobindin20 antibody
    • CPB II antibody
    • CPB-II antibody
    • CPBII antibody
    • Lipocortin VI antibody
    • LipocortinVI antibody
    • p68 antibody
    • p70 antibody
    • Protein III antibody
    • ProteinIII antibody
    see all

Anti-Annexin VI antibody 图像

  • All lanes : Anti-Annexin VI antibody (ab52221) at 1/500 dilution

    Lane 1 : extracts from 293 cells,
    Lane 2 : extracts from 293 cells, with immunizing peptide.


    Predicted band size : 75 kDa
    Observed band size : 75 kDa
  • IHC image of ab52221 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52221, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Annexin VI antibody (ab52221) at 1/1000 dilution + Recombinant Human Annexin VI protein (ab92968) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Anti-Annexin VI antibody (ab52221)参考文献

ab52221 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Of our Annexin VI antibodies, ab19416 is the most likely to cross-react with chicken (UniProt: P51901). The homologies of their immunogens with the chicken sequence is as follows: ab115811: 69% ab30126/ ab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"