Annexin V-PE-Cy5 Apoptosis Detection试剂盒(ab14159)

概述

  • 产品名称Annexin V-PE-Cy5 Apoptosis Detection试剂盒
    参阅全部 Annexin V 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Direct
  • 检测时间
    0h 10m
  • 产品概述

    Annexin V-PE-Cy5 Apoptosis Detection Kit (ab14159) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation. Results can be analyzed by flow cytometry or fluorescence microscopy.
    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用适用于: FM, Flow Cytmore details

性能

  • 功能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关Pregnancy loss, recurrent, 3
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 别名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

应用

Our Abpromise guarantee covers the use of ab14159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

实验方案

Annexin V-PE-Cy5 Apoptosis Detection Kit (ab14159)参考文献

ab14159 has not yet been referenced specifically in any publications.

Product Wall



We do not have any experimentaldata for theuse of this kitwith GFP, howeverI think this should be ok sincethe kit uses the tandem (PE-Cy5) for which the excitation wavelengthis 488 nm and the emission 670nm. This should not interfere with the...

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I'm very sorry to hear you are having problems with this kit and for the shortages you have experienced. I have arranged for 2 kits to be sent to you as soon as possible (order reference 80894) and apologise for the inconvenience. I also enclose the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"