Annexin V-PE Apoptosis Staining / Detection试剂盒(ab14155)


  • 产品名称
    Annexin V-PE Apoptosis Staining / Detection试剂盒
    参阅全部 Annexin V 试剂盒
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
  • 检测时间
    0h 10m
  • 产品概述

    Annexin V-PE Apoptosis Staining / Detection Kit (ab14155) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用
    适用于: FM, Flow Cytmore details


  • 功能
    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关
    Pregnancy loss, recurrent, 3
  • 序列相似性
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 别名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all


Our Abpromise guarantee covers the use of ab14155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Annexin V-PE Apoptosis Staining / Detection Kit 图像

  • Annexin V detected in 10e4 control Jurkat cells (black) or in cells treated with 50 μM H2O2 (Sigma) for 6 hours (red).


Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)参考文献

This product has been referenced in:
  • Alegret JM  et al. Data on the circulating levels of endothelial microparticles are elevated in patients with bicuspid aortic valve and are related to aortic dilation. Data Brief 8:666-9 (2016). Read more (PubMed: 27453924) »
  • Stolzing A  et al. Glucose-induced replicative senescence in mesenchymal stem cells. Rejuvenation Res 9:31-5 (2006). Read more (PubMed: 16608393) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

I have investigated our records for ab14155. I can confirm that this kit contains Annexin V-PE.

The datasheet has been updated with the correct information and I apologize for any confusion. (Please not...

Read More

Thanks for your enquiry.

The reagent that comes with this kit is not an antibody. It is PE/Cy5-labeled Annexin V protein. Annexin V will bind specifically to the membrane phospholipid phosphatidylserine, which in apoptotic cells has transloca...

Read More

The source of the Annexin V-PE Apoptosis Detection Kit informed me that the formulation of the buffer is confidential and therefore I am unable to compare it with the customer's home made buffer, I'm very sorry. The customer can buy more of the buff...

Read More

Thank you for your enquiry. Yes, ab14084 is the replacement buffer product for this kit. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.