Annexin V-PE Apoptosis Detection试剂盒With SYTOX (ab39674)

概述

  • 产品名称Annexin V-PE Apoptosis Detection试剂盒With SYTOX
    参阅全部 Annexin V 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Direct
  • 检测时间
    0h 10m
  • 产品概述

    Annexin V-PE Apoptosis Detection Kit with SYTOX (ab39674) includes Annexin V-PE, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeable to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-PE and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and PE filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-PE (Ex. 488 nm/Em. 578 nm).
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    The assay is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation.

  • 经测试应用适用于: Flow Cyt, FMmore details

性能

  • 功能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关Pregnancy loss, recurrent, 3
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 别名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

应用

Our Abpromise guarantee covers the use of ab39674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent dilution.
FM Use at an assay dependent concentration.

实验方案

Annexin V-PE Apoptosis Detection Kit With SYTOX (ab39674)参考文献

ab39674 has not yet been referenced specifically in any publications.

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