Annexin V-FITC Apoptosis Detection试剂(500X) (ab14082)

概述

性能

  • 功能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关Pregnancy loss, recurrent, 3
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 别名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

应用

Our Abpromise guarantee covers the use of ab14082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF Use 1µl for 105 cells.

Annexin V-FITC Apoptosis Detection Reagent (500X) 图像

  • Murine platelets were stimulated with CRP (5 µg/ml) in the presence of annexin V-fluorescein isothiocyanate for 10 minutes. The annexin V-positive population is indicated (AnV +ve).

实验方案

Annexin V-FITC Apoptosis Detection Reagent (500X) (ab14082)参考文献

This product has been referenced in:
  • Ghosh AS  et al. Hydrogen peroxide-induced apoptosis-like cell death in Entamoeba histolytica. Parasitol Int 59:166-72 (2010). Read more (PubMed: 20079879) »
  • Harper MT & Poole AW Protein kinase Ctheta negatively regulates store-independent Ca2+ entry and phosphatidylserine exposure downstream of glycoprotein VI in platelets. J Biol Chem 285:19865-73 (2010). Flow Cyt . Read more (PubMed: 20388711) »

See all 4 Publications for this product

Product Wall

Thank you for your inquiry.

I can confirm that ab14082 (5X Annexin V-FITC Apoptosis Detection Reagent) and ab14084 (10X Annexin V Binding Buffer) are the same reagents as in the kit ab14085(Annexin V-FITC Apoptosis Detection Kit).

P...

Read More

Thank you for your reply.

Both Annexin V-EGFP Apoptosis Detection Reagents (ab14152 and ab14082) contain a protocol booklet in their datasheets. These are the recommended protocols to use along with the Propidium Iodide reagent.

Ple...

Read More

Thank you for your enquiry and your interest in our products.

The concentration of this product (ab14082) is 0.15 mg/ml, which is equivalent to 0.15 µg/ul. This is added to 500 µl makes it 0.15 µg/500 µl, which is 0.3 ...

Read More

Thank you for contacting us for technical support, I'm sorry to hear you are experiencing problems with ab14082, I am trying to find out from the lab the function of the binding buffer and as soon as I find out I will forward to you this information. ...

Read More

Thank you for your enquiry. The associated concentration for the Annexin V- FITC conjugate in the Annexin V- FITC Apoptosis Detection Reagent Kit is 250 ug/ml. Please contact us again if you require further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"