Annexin V-Cy3 Apoptosis Detection试剂盒(ab14142)

概述

  • 产品名称Annexin V-Cy3 Apoptosis Detection试剂盒
    参阅全部 Annexin V 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Direct
  • 检测时间
    0h 10m
  • 产品概述

    Abcam's Annexin V-Cy3 Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用适用于: FM, Flow Cytmore details

性能

  • 功能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关Pregnancy loss, recurrent, 3
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 别名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

应用

Our Abpromise guarantee covers the use of ab14142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

实验方案

Annexin V-Cy3 Apoptosis Detection Kit (ab14142)参考文献

This product has been referenced in:
  • Xu P  et al. The different role of Notch1 and Notch2 in astrocytic gliomas. PLoS One 8:e53654 (2013). Flow Cyt . Read more (PubMed: 23349727) »
  • Zhou X  et al. Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status. Lab Invest 90:144-55 (2010). Read more (PubMed: 20048743) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us. This kit can only be used with fresh cells and tissue samples. Any process that leads to membrane damage like fixation and/or freeze-thaw cycles makes the sample incompatible with this kit. So unfortunately it cannot be...

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Thank you for your enquiry. Annexin V assay can only be used with fresh tissue sample. Simply incubate annexin V with the tissue piece, then visualize it using confocal microscopy. Frozen or fixed tissue are not suitable for the assay. For frozen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"