RabMAb

Anti-Annexin V抗体[EPR3980] (ab108194)

概述

  • 产品名称Anti-Annexin V抗体[EPR3980]
    参阅全部 Annexin V 一抗
  • 描述
    兔单克隆抗体[EPR3980] to Annexin V
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Annexin V aa 300 to the C-terminus. The exact sequence is proprietary.

  • 阳性对照
    • WB: HeLa, fetal kidney, fetal brain, Jurkat and JAR cell lysates ICC: HeLa cells; staurosporin-treated apoptotic HeLa cells
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 36 kDa.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/1000.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    • 疾病相关Pregnancy loss, recurrent, 3
    • 序列相似性Belongs to the annexin family.
      Contains 4 annexin repeats.
    • 结构域The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • 翻译后修饰S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Anchorin CII antibody
      • Annexin 5 antibody
      • Annexin A5 antibody
      • Annexin V antibody
      • Annexin-5 antibody
      • ANX A5 antibody
      • ANX5 antibody
      • ANXA5 antibody
      • ANXA5_HUMAN antibody
      • Calphobindin I antibody
      • CBP-I antibody
      • Endonexin II antibody
      • ENX2 antibody
      • Lipocortin V antibody
      • PAP I antibody
      • PAP-I antibody
      • Placental anticoagulant protein 4 antibody
      • Placental anticoagulant protein I antibody
      • PLACENTAL PROTEIN 4 antibody
      • Pp4 antibody
      • Thromboplastin inhibitor antibody
      • VAC-alpha antibody
      • Vascular anticoagulant-alpha antibody
      see all

    Anti-Annexin V antibody [EPR3980] 图像

    • Overlay histogram showing HeLa cells stained with ab108194 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108194, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Annexin V antibody [EPR3980] (ab108194)

      Lane 1 : HeLa cell lysate
      Lane 2 : Fetal kidney lysate
      Lane 3 : Fetal brain lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 36 kDa
    • Immunofluorescent staining of HeLa cells using 1/500 ab108194.
    • Immunofluorescent staining of staurosporin-treated apoptotic HeLa cells using 1/250 ab108194.

    Anti-Annexin V antibody [EPR3980] (ab108194)参考文献

    This product has been referenced in:
    • Ververis K & Karagiannis TC An atlas of histone deacetylase expression in breast cancer: fluorescence methodology for comparative semi-quantitative analysis. Am J Transl Res 4:24-43 (2012). ICC/IF ; Human . Read more (PubMed: 22347520) »

    See 1 Publication for this product

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