RabMAb

Anti-Annexin V抗体[EPR3979] (ab108321)

概述

  • 产品名称Anti-Annexin V抗体[EPR3979]
    参阅全部 Annexin V 一抗
  • 描述
    兔单克隆抗体[EPR3979] to Annexin V
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Annexin V.

  • 阳性对照
    • HeLa, JAR, and fetal kidney lysates; Human gastric tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    • 疾病相关Pregnancy loss, recurrent, 3
    • 序列相似性Belongs to the annexin family.
      Contains 4 annexin repeats.
    • 结构域The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • 翻译后修饰S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Anchorin CII antibody
      • Annexin 5 antibody
      • Annexin A5 antibody
      • Annexin V antibody
      • Annexin-5 antibody
      • ANX A5 antibody
      • ANX5 antibody
      • ANXA5 antibody
      • ANXA5_HUMAN antibody
      • Calphobindin I antibody
      • CBP-I antibody
      • Endonexin II antibody
      • ENX2 antibody
      • Lipocortin V antibody
      • PAP I antibody
      • PAP-I antibody
      • Placental anticoagulant protein 4 antibody
      • Placental anticoagulant protein I antibody
      • PLACENTAL PROTEIN 4 antibody
      • Pp4 antibody
      • Thromboplastin inhibitor antibody
      • VAC-alpha antibody
      • Vascular anticoagulant-alpha antibody
      see all

    Anti-Annexin V antibody [EPR3979] 图像

    • All lanes : Anti-Annexin V antibody [EPR3979] (ab108321) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : fetal kidney lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 36 kDa
    • Immunohistochemical analysis of Annexin V in paraffin embedded Human gastric tissue, using ab108321 at a 1/100 dilution.

    Anti-Annexin V antibody [EPR3979] (ab108321)参考文献

    This product has been referenced in:
    • Liu B  et al. Altered protein expression in gestational diabetes mellitus placentas provides insight into insulin resistance and coagulation/fibrinolysis pathways. PLoS One 7:e44701 (2012). WB, IHC-P ; Human . Read more (PubMed: 22970290) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
    Sample Human Tissue sections (human colon - muscle cells)
    Specification human colon - muscle cells
    Permeabilization Yes - 0.2% Triton X-100 and PBS
    Fixative Formaldehyde
    Username

    Mrs. Maria Cecilia Ybanez

    Verified customer

    提交于 Mar 11 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% T ween 20, pH 6.0)
    Sample Human Tissue sections (human colon)
    Specification human colon
    Permeabilization Yes - 0.2% Triton X-100 and PBS
    Fixative Formaldehyde
    Username

    Dr. Steffen Rickelt

    Verified customer

    提交于 Nov 06 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Sep 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"