概述

  • 产品名称
    Anti-Annexin V抗体[AN5]
    参阅全部 Annexin V 一抗
  • 描述
    小鼠单克隆抗体[AN5] to Annexin V
  • 宿主
    Mouse
  • 经测试应用
    适用于: Indirect ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human annexin V

  • 阳性对照
    • HeLa total cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab49823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能
    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关
    Pregnancy loss, recurrent, 3
  • 序列相似性
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anchorin CII antibody
    • Annexin 5 antibody
    • Annexin A5 antibody
    • Annexin V antibody
    • Annexin-5 antibody
    • ANX A5 antibody
    • ANX5 antibody
    • ANXA5 antibody
    • ANXA5_HUMAN antibody
    • Calphobindin I antibody
    • CBP-I antibody
    • Endonexin II antibody
    • ENX2 antibody
    • Lipocortin V antibody
    • PAP I antibody
    • PAP-I antibody
    • Placental anticoagulant protein 4 antibody
    • Placental anticoagulant protein I antibody
    • PLACENTAL PROTEIN 4 antibody
    • Pp4 antibody
    • Thromboplastin inhibitor antibody
    • VAC-alpha antibody
    • Vascular anticoagulant-alpha antibody
    see all

图片

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Annexin V knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab49823 observed at 35 kDa. Red - loading control, ab176560, observed at 50 kDa.

    ab49823 was shown to specifically react with Annexin V in wild-type cells as signal was lost in Annexin V knockout cells. Wild-type and Annexin V knockout samples were subjected to SDS-PAGE. Ab49823 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 0.5 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Annexin V antibody [AN5] (ab49823) at 0.5 µg/ml + HeLa total cell extract

    Secondary
    Goat Anti-Mouse, Alkaline Phosphatase conjugate

    Predicted band size: 38 kDa
    Observed band size: 29 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Haedicke K  et al. Multiplexed in vivo fluorescence optical imaging of the therapeutic efficacy of photodynamic therapy. Biomaterials 34:10075-83 (2013). ICC/IF . Read more (PubMed: 24050876) »

See 1 Publication for this product

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