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Our Abpromise guarantee covers the use of ab49823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin V knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab49823 observed at 35 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab49823 was shown to specifically react with Annexin V in wild-type cells as signal was lost in Annexin V knockout cells. Wild-type and Annexin V knockout samples were subjected to SDS-PAGE. Ab49823 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 0.5 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"