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Recombinant full length protein, corresponding to amino acids 1-321 of Human Annexin V
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab108194 as a replacement.
Our Abpromise guarantee covers the use of ab54775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"