Anti-Annexin V/ANXA5抗体[1F4-1A5] (ab54775)
Key features and details
- Mouse monoclonal [1F4-1A5] to Annexin V/ANXA5
- Suitable for: IP, WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Annexin V/ANXA5抗体[1F4-1A5]
参阅全部 Annexin V/ANXA5 一抗 -
描述
小鼠单克隆抗体[1F4-1A5] to Annexin V/ANXA5 -
宿主
Mouse -
经测试应用
适用于: IP, WB, ICC/IF, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Annexin V/ANXA5 aa 1-321.
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常规说明
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
1F4-1A5 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54775于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
疾病相关
Pregnancy loss, recurrent, 3 -
序列相似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
结构域
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻译后修饰
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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数据库链接
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
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别名
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
see all
图片
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ab54775 Western Blot analysis of ANXA5/ANXA5 expression in Hela cells.
This image was generated using the ascites version of the product.
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Annexin V/ANXA5 antibody (ab54775) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
This image was generated using the ascites version of the product.
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ab54775 at 10 ug/ml staining Annexin V/ANXA5 in human Hela cells by Immunocytochemistry / Immunofluorescence.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab54775 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54775, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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Immunoprecipitation of ANXA5 transfected lysate using ab54775 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with a ANXA5 rabbit polyclonal antibody.
This image was generated using the ascites version of the product.
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Western Blot analysis of ANXA5 expression in transfected 293T cell line by ab54775.
Lane 1: ANXA5 transfected lysate(35.9 KDa).
Lane 2: Non-transfected lysate.This image was generated using the ascites version of the product.
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ab54775 staining Annexin V/ANXA5 in HeLa cells treated with pentoxifylline (ab120725), by ICC/IF. Increase of Annexin V/ANXA5 correlates with increased concentration of pentoxifylline, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120725 (pentoxifylline) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab54775 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab54775 被引用在 20 文献中.
- Martínez-Lagunas K et al. In vivo detection of programmed cell death during mouse heart development. Cell Death Differ 27:1398-1414 (2020). PubMed: 31570857
- Zietzer A et al. The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles. J Extracell Vesicles 9:1786967 (2020). PubMed: 32944175
- Bartolome F et al. Annexin A5 prevents amyloid-ß-induced toxicity in choroid plexus: implication for Alzheimer's disease. Sci Rep 10:9391 (2020). PubMed: 32523019
- Bollinger AL et al. Annexin V expression on CD4+ T cells with regulatory function. Immunology 159:205-220 (2020). PubMed: 31642515
- Hesse R et al. Comparative profiling of the synaptic proteome from Alzheimer's disease patients with focus on the APOE genotype. Acta Neuropathol Commun 7:214 (2019). PubMed: 31862015