概述

  • 产品名称
    Anti-Annexin V抗体
    参阅全部 Annexin V 一抗
  • 描述
    兔多克隆抗体to Annexin V
  • 经测试应用
    适用于: Flow Cyt, WB, IHC-FoFr, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Annexin V.
    (Peptide available as ab52111)

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.

性能

应用

Our Abpromise guarantee covers the use of ab14196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 4 µg/ml. Can be blocked with Annexin V Blocking peptide (ab52111).
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20086251

靶标

  • 功能
    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关
    Pregnancy loss, recurrent, 3
  • 序列相似性
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anchorin CII antibody
    • Annexin 5 antibody
    • Annexin A5 antibody
    • Annexin V antibody
    • Annexin-5 antibody
    • ANX A5 antibody
    • ANX5 antibody
    • ANXA5 antibody
    • ANXA5_HUMAN antibody
    • Calphobindin I antibody
    • CBP-I antibody
    • Endonexin II antibody
    • ENX2 antibody
    • Lipocortin V antibody
    • PAP I antibody
    • PAP-I antibody
    • Placental anticoagulant protein 4 antibody
    • Placental anticoagulant protein I antibody
    • PLACENTAL PROTEIN 4 antibody
    • Pp4 antibody
    • Thromboplastin inhibitor antibody
    • VAC-alpha antibody
    • Vascular anticoagulant-alpha antibody
    see all

Anti-Annexin V antibody 图像

  • ab14196 staining Annexin V in mouse embryonic 15 day brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, ermeablized with 0.5% Triton X-100 in PBS, blocked with 10% serum for 1 hour at 25°C and antigen retrieval was by heat mediation in citrate buffer, pH 6. The sample was incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit polyclonal (1/700) was used as the secondary antibody.

    See Abreview

  • Anti-Annexin V antibody (ab14196) + HeLa cell lysate
  • ab14196 staining cells from a mouse organ of Corti (whole mount) by ICC/IF.  The tissue was PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% goat serum, 2% BSA for 1 hour at 25°C.  The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 25°C.  A Cy3® conjugated sheep anti-rabbit IgG antibody, diluted 1/200 was used as the secondary. Alexa Fluor 488® - Phalloidin was used to label the actin cytoskeleton and DAPI was used to label nuclei.

    See Abreview

  • ab14196 staining cells from a human pancreatic cell line by ICC/IF.  Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary.

    See Abreview

  • IHC image of Annexin V staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14196, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Annexin V antibody (ab14196)参考文献

This product has been referenced in:
  • Kim OH  et al. Impaired phagocytosis of apoptotic cells causes accumulation of bone marrow-derived macrophages in aged mice. BMB Rep 50:43-48 (2017). Read more (PubMed: 27866511) »
  • Hefter D  et al. Amyloid Precursor Protein Protects Neuronal Network Function after Hypoxia via Control of Voltage-Gated Calcium Channels. J Neurosci 36:8356-71 (2016). Read more (PubMed: 27511009) »

See all 25 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Jaculus jaculus Tissue sections (Skin)
Permeabilization
Yes - 0.1% Triton
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Mai Tran

Verified customer

提交于 Nov 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huamn)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
alpha synuclein
Specification
huamn
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Sehee Oh

Verified customer

提交于 Jul 13 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (HBMEC (Human Brain Microvascular Endothelial Cell))
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
HBMEC (Human Brain Microvascular Endothelial Cell)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 31 2015

Application
Western blot
Sample
Rat Cell lysate - whole cell (Pericytes)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Pericytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (bEnd.3)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
bEnd.3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 07 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample
Mouse Tissue sections (Embryonic 15 day brain)
Specification
Embryonic 15 day brain
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 23 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (lumbar spinal cord, 25 micron)
Specification
lumbar spinal cord, 25 micron
Permeabilization
Yes - 0.5% Triton X100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 04 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Human Tissue sections (human colon - muscle cells)
Specification
human colon - muscle cells
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Melanoma B16F10 tumor)
Specification
Melanoma B16F10 tumor
Fixative
4% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: autoclaved for 10min at 121 Celcius (10mM Sodium Citrate, pH 6.0)
Permeabilization
Yes - 0.1% SDS
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

提交于 May 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Melanoma B16F10 tumor)
Loading amount
30 µg
Specification
Melanoma B16F10 tumor
Treatment
5gy Irradiation
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

提交于 Mar 15 2013

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