概述

  • 产品名称
    Anti-Annexin IV抗体
    参阅全部 Annexin IV 一抗
  • 描述
    兔多克隆抗体to Annexin IV
  • 经测试应用
    适用于: IHC-Fr, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within Synthetic peptide: GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS TIGRDLIDDL KSELSGNFEQ , corresponding to N terminal amino acids 31-80 of Human Annexin IV

  • 阳性对照
    • WB: Placental lysate. IHC-P: Human intestine.

性能

应用

Our Abpromise guarantee covers the use of ab33009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100.
ELISA 1/1.5625e+006.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
  • 序列相似性
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 35 beta calcimedin antibody
    • 35-beta calcimedin antibody
    • AIV antibody
    • Annexin 4 antibody
    • Annexin A4 antibody
    • Annexin IV (placental anticoagulant protein II) antibody
    • Annexin IV antibody
    • Annexin IV placental anticoagulant protein II antibody
    • Annexin-4 antibody
    • AnnexinA4 antibody
    • AnnexinIV antibody
    • ANX 4 antibody
    • ANX A4 antibody
    • ANX4 antibody
    • ANXA 4 antibody
    • ANXA4 antibody
    • ANXA4 protein antibody
    • ANXA4_HUMAN antibody
    • Carbohydrate binding protein P33/P41 antibody
    • Carbohydrate-binding protein p33/p41 antibody
    • Chromobindin 4 antibody
    • Chromobindin-4 antibody
    • Chromobindin4 antibody
    • DKFZp686H02120 antibody
    • Endonexin I antibody
    • EndonexinI antibody
    • Lipocortin IV antibody
    • LipocortinIV antibody
    • MGC75105 antibody
    • OTTHUMP00000160052 antibody
    • OTTHUMP00000202207 antibody
    • P32.5 antibody
    • P33/41 antibody
    • PAP II antibody
    • PAP-II antibody
    • PAPII antibody
    • PIG 28 antibody
    • PIG28 antibody
    • Placental anticoagulant protein II antibody
    • PP4 X antibody
    • PP4-X antibody
    • PP4X antibody
    • Proliferation inducing gene 28 antibody
    • Proliferation inducing protein 28 antibody
    • Protein II antibody
    • Xanx-4 antibody
    • ZAP 36 antibody
    • ZAP36 antibody
    see all

图片

  • Anti-Annexin IV antibody (ab33009) at 1.25 µg/ml + Placental lysate

    Secondary
    HRP conjugated anti Rabbit IgG 1/50000 - 1/100000

    Predicted band size : 36 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelled at Annexin IV with ab33009 at 4-8µg/ml. Positively labelled cells (indicated by arrows) are epithelial cells of intestinal villi. Magnification: 400X.

文献

This product has been referenced in:
  • Choi CH  et al. Prognostic significance of annexin A2 and annexin A4 expression in patients with cervical cancer. BMC Cancer 16:448 (2016). Read more (PubMed: 27402115) »
  • Chen W  et al. Overexpression of annexin A4 indicates poor prognosis and promotes tumor metastasis of hepatocellular carcinoma. Tumour Biol 37:9343-55 (2016). Read more (PubMed: 26779633) »

See all 7 Publications for this product

客户评价及客户问答

Sorry for the delay in getting back to you.

I can now provide further advice for the customer. Whilst the antibody anti-Annexin IV (ab33009) will not be able to detect the fusion construct the customer has been expressing (with ANXA4 reidues ...

Read More

Thank you very much for this information and I am sorry for the delay in getting back to you. I have been away from the office.

With this information provided, I now think I know why the experiment has not been working for the customer. The a...

Read More

Thank you very much for sharing that information with me. It has been very helpful.

In order to ascertain if the antibody Anti-Annexin IV antibody (ab33009) is likely to detect the protein you have been expressing, could you please let me kno...

Read More

Thank you for providing that information.

In order to understand what might be expected could you please answer the following:

1. Was the plasmid constructed by yourself?

2. What size protein was obtained following purific...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibodyhas not been providing you with the expected results.

The details provided will enable us to investigate this case and will provide us with vital i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Small intestinal epithelial cells)
Specification
Small intestinal epithelial cells
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Abeer Hammoudi

Verified customer

提交于 May 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (small intestine -epithelial cells extract)
Loading amount
20 µg
Specification
small intestine -epithelial cells extract
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Abeer Hammoudi

Verified customer

提交于 May 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (cartilage)
Specification
cartilage
Fixative
Methanol
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

提交于 Aug 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册