Annexin A2 overexpression 293T裂解液(全细胞) (ab94080)

概述

  • 产品名称Annexin A2 overexpression 293T裂解液(全细胞)
  • 描述
    Annexin A2 overexpression裂解液
  • 常规说明ab94080 is a 293T cell transfected lysate in which Human Annexin II has been transiently over-expressed using a pCMV-Annexin II plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. PTM: Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.

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应用

Our Abpromise guarantee covers the use of ab94080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Annexin A2 overexpression 293T lysate (whole cell) 图像

  • ab94080 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Annexin A2 antibody (ab54771) at 1/500 dilution

    Lane 1 : Annexin II 293T Transfected Lysate - Positive Control (ab94080)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

Annexin A2 overexpression 293T lysate (whole cell) (ab94080)参考文献

ab94080 has not yet been referenced specifically in any publications.

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