The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 38 kDa.
1/10 - 1/50.
Use a concentration of 10 µg/ml.
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
Belongs to the annexin family. Contains 4 annexin repeats.
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated.
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
Western blot - Annexin II antibody - N-terminal (ab75932)
Anti-Annexin A2 antibody - N-terminal (ab75932) at 1/50 dilution + mouse lung lysate at 35 µg
Predicted band size: 38 kDa Observed band size: 38 kDa Additional bands at: 45 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Annexin II antibody - N-terminal(ab75932)
ab75932 at 1/50 dilution staining Annexin II - N-terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-Annexin II antibody - N-terminal (ab75932)
ICC/IF image of ab75932 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75932, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.