概述

  • 产品名称Anti-Annexin A2抗体
    参阅全部 Annexin A2 一抗
  • 描述
    山羊多克隆抗体to Annexin A2
  • 特异性This antibody is expected to recognise both reported isoforms. NP_004030.1, NP_001002857.1and NP_001129487.1 are varients of isoform 2.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide:

    STVHEILCKLSLEGD

    , corresponding to N terminal amino acids 2-16 of Human Annexin II.

  • 阳性对照
    • HeLa and A549 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab2242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide (ab22880).

靶标

  • 功能Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Anti-Annexin A2 antibody 图像

  • Anti-Annexin A2 antibody (ab2242) at 0.5 µg/ml + A431 lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 38 kDa

Anti-Annexin A2 antibody (ab2242)参考文献

This product has been referenced in:
  • Ribeiro-Oliveira A  et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . Read more (PubMed: 18701577) »
  • Glebov OO  et al. Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells. Nat Cell Biol 8:46-54 (2006). Read more (PubMed: 16341206) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (pig brain lysate and pig endothelial cell line lys)
Loading amount 10 µg
Specification pig brain lysate and pig endothelial cell line lys
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
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Abcam user community

Verified customer

提交于 Aug 04 2006

Thank you for your enquiry. To our knowledge, these antibodies have yet to be tested in IHC-P. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you g...

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Thank you for your email. It does sound like the problem was with this primary, especially as you successfully used a different annexin II primary under the same conditions. I have instructed our accounting department to issue you a credit for your ord...

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Thank you for your enquiry and patience, and I'm sorry to hear that you are experiencing difficulty with ab2242. We have not had any complaints regarding this antibody, and so there is a possibility that the vial you received went off during the shippi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"