概述

  • 产品名称Anti-Annexin A1抗体
    参阅全部 Annexin A1 一抗
  • 描述
    小鼠多克隆抗体to Annexin A1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human Annexin A1 protein (NP_000691.1).

  • 阳性对照
    • Human spleen tissue lysate, Annexin A1 transfected 293T cell lysate, Human endometrium tissue and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method in 1X citrate buffer (pH 6.0) before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
    • 序列相似性Belongs to the annexin family.
      Contains 4 annexin repeats.
    • 结构域A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • 翻译后修饰Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
    • 细胞定位Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Annexin 1 antibody
      • Annexin A1 antibody
      • Annexin I (lipocortin I) antibody
      • Annexin I antibody
      • Annexin-1 antibody
      • AnnexinA1 antibody
      • AnnexinI antibody
      • ANX 1 antibody
      • ANX A1 antibody
      • ANX1 antibody
      • ANXA 1 antibody
      • ANXA1 antibody
      • ANXA1 protein antibody
      • ANXA1_HUMAN antibody
      • Calpactin 2 antibody
      • Calpactin II antibody
      • Calpactin-2 antibody
      • CalpactinII antibody
      • Chromobindin 9 antibody
      • Chromobindin-9 antibody
      • Chromobindin9 antibody
      • HGNC:533 antibody
      • Lipocortin 1 antibody
      • Lipocortin I antibody
      • Lipocortin1 antibody
      • LipocortinI antibody
      • LPC 1 antibody
      • LPC1 antibody
      • p35 antibody
      • Phospholipase A2 inhibitory protein antibody
      see all

    Anti-Annexin A1 antibody 图像

    • Anti-Annexin A1 antibody (ab88865) at 1 µg/ml + human spleen tissue lysate at 50 µg

      Predicted band size : 39 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-Annexin A1 antibody (ab88865) at 1 µg/ml

      Lane 1 : Annexin A1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 39 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    • ab88865, at 3 µg/ml staining Annexin A1 in formalin-fixed, paraffin-embedded human endometrium by Immunohistochemistry.
    • ab88865, at 10 µg/ml staining Annexin A1 in HeLa cells by Immunofluorescence.

    Anti-Annexin A1 antibody (ab88865)参考文献

    ab88865 has not yet been referenced specifically in any publications.

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