概述

  • 产品名称Anti-Annexin A1抗体
    参阅全部 Annexin A1 一抗
  • 描述
    小鼠单克隆抗体to Annexin A1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full-length recombinant Human protein (AAH01275) with proprietary tag

  • 阳性对照
    • Lysate from HepG2 cells or placenta tissue

性能

应用

Our Abpromise guarantee covers the use of ab88060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 39 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • 细胞定位Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Anti-Annexin A1 antibody 图像



  • Predicted band size : 39 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Annexin A1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

     

    Lanes 1 - 4: Merged signal (red and green). Green - ab88060 observed at 38 kDa. Red - loading control, ab176560, observed at 50 kDa.

     

    ab88060 was shown to specifically react with Annexin A1 when Annexin A1 knockout samples were used. Wild-type and Annexin A1 knockout samples were subjected to SDS-PAGE. Ab88060 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-Annexin A1 antibody (ab88060) at 5 µg/ml + immunogen (recombinant full length tagged protein) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 39 kDa
    MW of tag alone: 26kDa
  • Anti-Annexin A1 antibody (ab88060) at 5 µg/ml + Human placenta lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab88060) at 5 µg/ml + HepG2 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 39 kDa
  • All lanes : Anti-Annexin A1 antibody (ab88060) at 5 µg/ml

    Lane 1 : transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 39 kDa

Anti-Annexin A1 antibody (ab88060)参考文献

ab88060 has not yet been referenced specifically in any publications.

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