Anti-Annexin A1抗体(ab81528)

概述

  • 产品名称Anti-Annexin A1抗体
    参阅全部 Annexin A1 一抗
  • 描述
    山羊多克隆抗体to Annexin A1
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide:

    CQAILDETKGDYEK

    , corresponding to C terminal amino acids 324-337 of Human Annexin A1 according to NP_000691.1

  • 阳性对照
    • A431 lysate Rat spleen

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab81528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.

Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1).

靶标

Anti-Annexin A1 antibody 图像

  • All lanes : Anti-Annexin A1 antibody (ab81528) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 1 µg/ml + Pig spleen lysate at 35 µg

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 1 µg/ml (for 1 hour) + Rat spleen lysate in RIPA buffer at 35 µg

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 0.5 µg/ml (for 1 hour) + A431 lysate in RIPA buffer at 35 µg

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-Annexin A1 antibody (ab81528)参考文献

ab81528 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - other (protein lysate from lymph node)
Loading amount 30 µg
Specification protein lysate from lymph node
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Angela Moreno

Verified customer

提交于 Mar 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"