Anti-Annexin A1抗体(ab81528)

概述

  • 产品名称
    Anti-Annexin A1抗体
    参阅全部 Annexin A1 一抗
  • 描述
    山羊多克隆抗体to Annexin A1
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide:

    CQAILDETKGDYEK

    , corresponding to C terminal amino acids 324-337 of Human Annexin A1 according to NP_000691.1

  • 阳性对照
    • A431 lysate Rat spleen

性能

应用

Our Abpromise guarantee covers the use of ab81528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.

Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1).

靶标

图片

  • All lanes : Anti-Annexin A1 antibody (ab81528) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 1 µg/ml + Pig spleen lysate at 35 µg

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 1 µg/ml (for 1 hour) + Rat spleen lysate in RIPA buffer at 35 µg

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 0.5 µg/ml (for 1 hour) + A431 lysate in RIPA buffer at 35 µg

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab81528 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - other (protein lysate from lymph node)
Loading amount
30 µg
Specification
protein lysate from lymph node
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Angela Moreno

Verified customer

提交于 Mar 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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