The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Belongs to the annexin family. Contains 4 annexin repeats.
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
ab115770, at 4 ug/ml, staining Annexin A1 in Formalin fixed, Paraffin embedded Human placenta by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115770, at 4 ug/ml, staining Annexin A1 in Formalin fixed, Paraffin embedded Human prostate by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Western blot - Anti-Annexin A1 antibody (ab115770)
Anti-Annexin A1 antibody (ab115770) at 0.5 µg/ml + A431 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Primary incubation was 1 hour. Detected by chemiluminescence