概述

  • 产品名称Anti-Annexin A1抗体[6E4/3]
    参阅全部 Annexin A1 一抗
  • 描述
    小鼠单克隆抗体[6E4/3] to Annexin A1
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Mixture of native membrane proteins from human bone tissues. This includes native whole annexin proteins.

性能

应用

Our Abpromise guarantee covers the use of ab4635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 38.5 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • 细胞定位Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Anti-Annexin A1 antibody [6E4/3] 图像

  • Ab4635 staining Human normal esophagus. Staining is localized to the nucleus.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Annexin A1 antibody [6E4/3] (ab4635)参考文献

This product has been referenced in:
  • Behrouz GF  et al. Presence of auto-antibody against two placental proteins, annexin A1 and vitamin D binding protein, in sera of women with pre-eclampsia. J Reprod Immunol 99:10-6 (2013). WB ; Human . Read more (PubMed: 23830177) »

See 1 Publication for this product

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