概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to ANKS1A
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-460 of Human ANKS1A

  • 阳性对照
    • ANKS1A transfected 293T cell lysate. HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
  • 应用说明
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 123 kDa).

    Note: This product requires purification with Protein A prior to ICC/IF.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      May play a negative role in growth factor receptor signaling pathways.
    • 组织特异性
      Widely expressed (at protein level).
    • 序列相似性
      Contains 6 ANK repeats.
      Contains 1 PID domain.
      Contains 2 SAM (sterile alpha motif) domains.
    • 翻译后修饰
      Phosphorylated on tyrosine residues in response to EGF and PDGF.
    • 细胞定位
      Cytoplasm. Cytoplasmic before and after growth factor treatment.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ANKS 1 antibody
      • ANKS 1A antibody
      • ANKS1 antibody
      • ANKS1A antibody
      • Ankyrin repeat and SAM domain containing 1 antibody
      • Ankyrin repeat and SAM domain containing protein 1A antibody
      • Ankyrin repeat and SAM domain-containing protein 1A antibody
      • Ankyrin repeat and sterile alpha motif domain containing 1A antibody
      • ANS1A_HUMAN antibody
      • KIAA0229 antibody
      • MGC42354 antibody
      • Odin antibody
      see all

    Anti-ANKS1A antibody 图像

    • All lanes : Anti-ANKS1A antibody (ab67533) at 1/500 dilution

      Lane 1 : ANKS1A transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 123 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of ANKS1A in HeLa cells using purified ab67533 at 10 ug/ml.

    Anti-ANKS1A antibody (ab67533)参考文献

    ab67533 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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