Ankyrin repeat domain-containing protein 50 antibody
Western blot - ANKRD50 antibody (ab108219)
All lanes : Anti-ANKRD50 antibody (ab108219) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 156 kDa
Exposure time: 30 seconds
Immunoprecipitation - ANKRD50 antibody (ab108219)
ab108219 at 1 µg/ml detecting ANKRD50 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of ANKRD50
Lane 2: ab108219 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds