概述

  • 产品名称Anti-ANKRD35抗体
  • 描述
    兔多克隆抗体to ANKRD35
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: QLREELAAVW REKDAARGAL SRPVMEGALG TPRAEAAAAA WEKMEARLER VLARLEWAKA GLQVKPEVPS QESREGALKA APGSIKQDEE KEKRVPGA, corresponding to amino acids 494-591 of Human ANKRD35.

  • 阳性对照
    • RT-4 and U251-MG cell lysates; Human tonsil lysate; Human nasopharynx tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-ANKRD35 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab122279 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-ANKRD35 antibody (ab122279) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U251-MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab122279, at 1/250 dilution, staining ANKRD35 in paraffin-embedded Human nasopharynx tissue by Immunohistochemistry.

Anti-ANKRD35 antibody (ab122279)参考文献

ab122279 has not yet been referenced specifically in any publications.

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