Anti-Ankrd26抗体(ab47984)
Key features and details
- Rabbit polyclonal to Ankrd26
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Ankrd26抗体
参阅全部 Ankrd26 一抗 -
描述
兔多克隆抗体to Ankrd26 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Cow -
免疫原
Synthetic peptide:
MKKIFGFRSKGPSPL
, corresponding to amino acids 1-15 of Mouse Ankrd26 -
阳性对照
- MES cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 196 kDa.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 196 kDa. |
靶标
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序列相似性
Contains 5 ANK repeats. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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数据库链接
- Entrez Gene: 526041 Cow
- Entrez Gene: 22852 Human
- Entrez Gene: 312667 Rat
- Omim: 610855 Human
- SwissProt: Q9UPS8 Human
- Unigene: 361041 Human
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别名
- Ankrd26 antibody
- Ankyrin repeat domain 26 antibody
- Ankyrin repeat domain-containing protein 26 antibody
see all
图片
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Lanes 2-10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution
Lane 1 : BLANK
Lane 2 : MES cell lysate - 80µg
Lane 3 : MES cell lysate - 40µg
Lane 4 : 293T-ANKRD26
transfected cell lysate - 20µg
Lane 5 : control 293T cell
lysate - 20µg
Lane 6 : BSA-ANKRD26 conjugate - 20ng
Lane 7 : BSA - 500ng
Lane 8 : BSA - 100ng
Lane 9 : BSA - 20ng
Lane 10 : Protein standards
Secondary
Lanes 2-10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution
Predicted band size: 196 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. An observed band of ~81kDa has been obtained due to a fragment of Ankrd26 being used instead of the full length version.
数据表及文件
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Datasheet download
文献 (1)
ab47984 被引用在 1 文献中.
- Cardamone MD et al. GPS2/KDM4A pioneering activity regulates promoter-specific recruitment of PPAR?. Cell Rep 8:163-76 (2014). Mouse . PubMed: 24953653