Anti-ANKRD25抗体(ab99351)

概述

  • 产品名称Anti-ANKRD25抗体
    参阅全部 ANKRD25 一抗
  • 描述
    兔多克隆抗体to ANKRD25
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 550 - 600 of Human ANKRD25 (NP_056308.2)

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, Tris buffered saline, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG

相关产品

应用

Our Abpromise guarantee covers the use of ab99351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
IP Use a concentration of 2 - 5 µg/ml.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性ANKRD25 contains 5 ANK repeats. It is strongly expressed in cervix, colon, heart, kidney and lung.
  • 细胞定位Cytoplasm. Mitochondrion.
  • 数据库链接
  • 别名
    • ANKRD25 antibody
    • Ankyrin repeat domain 25 antibody
    • Ankyrin repeat domain containing protein 25 antibody
    • DKFZp434N161 antibody
    • FLJ20004 antibody
    • KANK 2 antibody
    • KANK2 antibody
    • KIAA1518 antibody
    • Kidney ankyrin repeat containing protein 2 antibody
    • KN motif and ankyrin repeat domain containing protein 2 antibody
    • KN motif and ankyrin repeat domains 2 antibody
    • Matrix remodeling associated protein 3 antibody
    • Matrix remodelling associated 3 antibody
    • MGC119707 antibody
    • MXRA3 antibody
    • SIP antibody
    • SRC 1 interacting protein antibody
    • SRC interacting protein antibody
    • SRC1 interacting protein antibody
    see all

Anti-ANKRD25 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD25 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ANKRD25 antibody (ab99351) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 91 kDa


    Exposure time : 3 minutes
  • Detection of Human ANKRD25 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). ANKRD25 was also immunoprecipitated by another rabbit anti-ANKRD25 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.

Anti-ANKRD25 antibody (ab99351)参考文献

ab99351 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (primary myometrial cells)
Gel Running Conditions Reduced Denaturing (7% Bis-Tris)
Loading amount 20 µg
Specification primary myometrial cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 May 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"