Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 550 - 600 of Human ANKRD25 (NP_056308.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS, Tris buffered saline, pH 7-8
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
Use a concentration of 2 - 5 µg/ml.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ANKRD25 contains 5 ANK repeats. It is strongly expressed in cervix, colon, heart, kidney and lung.
Ankyrin repeat domain 25 antibody
Ankyrin repeat domain containing protein 25 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD25 antibody (ab99351)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD25 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-ANKRD25 antibody (ab99351)
All lanes : Anti-ANKRD25 antibody (ab99351) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique. Predicted band size: 91 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-ANKRD25 antibody (ab99351)
Detection of Human ANKRD25 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). ANKRD25 was also immunoprecipitated by another rabbit anti-ANKRD25 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.
has not yet been referenced specifically in any publications.
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