Anti-ANKRD25抗体(ab99351)

概述

  • 产品名称
    Anti-ANKRD25抗体
    参阅全部 ANKRD25 一抗
  • 描述
    兔多克隆抗体to ANKRD25
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 550 - 600 of Human ANKRD25 (NP_056308.2)

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, Tris buffered saline, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab99351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
IP Use a concentration of 2 - 5 µg/ml.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性
    ANKRD25 contains 5 ANK repeats. It is strongly expressed in cervix, colon, heart, kidney and lung.
  • 细胞定位
    Cytoplasm. Mitochondrion.
  • 数据库链接
  • 别名
    • ANKRD25 antibody
    • Ankyrin repeat domain 25 antibody
    • Ankyrin repeat domain containing protein 25 antibody
    • DKFZp434N161 antibody
    • FLJ20004 antibody
    • KANK 2 antibody
    • KANK2 antibody
    • KIAA1518 antibody
    • Kidney ankyrin repeat containing protein 2 antibody
    • KN motif and ankyrin repeat domain containing protein 2 antibody
    • KN motif and ankyrin repeat domains 2 antibody
    • Matrix remodeling associated protein 3 antibody
    • Matrix remodelling associated 3 antibody
    • MGC119707 antibody
    • MXRA3 antibody
    • SIP antibody
    • SRC 1 interacting protein antibody
    • SRC interacting protein antibody
    • SRC1 interacting protein antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD25 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ANKRD25 antibody (ab99351) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 91 kDa


    Exposure time : 3 minutes
  • Detection of Human ANKRD25 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). ANKRD25 was also immunoprecipitated by another rabbit anti-ANKRD25 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.

文献

ab99351 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (primary myometrial cells)
Gel Running Conditions
Reduced Denaturing (7% Bis-Tris)
Loading amount
20 µg
Specification
primary myometrial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册