Anti-ANKRD17抗体(ab85726)
Key features and details
- Rabbit polyclonal to ANKRD17
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ANKRD17抗体 -
描述
兔多克隆抗体to ANKRD17 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide, corresponding to a region between residue 775 and 825 of Human ANKRD17 (NP_115593.3).
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阳性对照
- Whole cell lysate from HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85726于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 274 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 274 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Could play pivotal roles in cell cycle and DNA regulation (PubMed:19150984). Involved in innate immune defense against viruse by positively regulating the viral dsRNA receptors DDX58 and IFIH1 signaling pathways (PubMed:22328336). Involves in NOD2- and NOD1-mediated responses to bacteria suggesting a role in innate antibacterial immune pathways too (PubMed:23711367). Target of enterovirus 71 which is the major etiological agent of HFMD (hand, foot and mouth disease) (PubMed:17276651). Could play a central role for the formation and/or maintenance of the blood vessels of the circulation system. -
组织特异性
Ubiquitously expressed. -
序列相似性
Contains 25 ANK repeats.
Contains 1 KH domain. -
翻译后修饰
Phosphorylated by CDK2. -
细胞定位
Cytoplasm. Nucleus. Detected around the nucleolus. Localized on chromatin in a cell cycle-dependent manner. - Information by UniProt
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数据库链接
- Entrez Gene: 26057 Human
- Entrez Gene: 81702 Mouse
- Entrez Gene: 289521 Rat
- SwissProt: O75179 Human
- SwissProt: Q99NH0 Mouse
- Unigene: 601206 Human
- Unigene: 733994 Human
- Unigene: 743705 Human
see all -
别名
- ANKRD 17 antibody
- ANKRD17 antibody
- Ankyrin repeat domain protein 17 antibody
see all
图片
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All lanes : Anti-ANKRD17 antibody (ab85726) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 274 kDa
Observed band size: 274 kDa
Additional bands at: 268 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of Human ANKRD17 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85726 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab85726 被引用在 1 文献中.
- Liu Y et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409