概述

  • 产品名称Anti-ANKRD12抗体
  • 描述
    兔多克隆抗体to ANKRD12
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: NEERDSDTDS DPGHTSENWG ERLISSYRTY SEKEGPEKKK TKKEAGNKKS, corresponding to N terminal amino acids 71-120 of Human ANKRD12

  • 阳性对照
    • HepG2 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab66309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 236 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性Ankyrin repeats containing cofactors may recruit histone deacetylases to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ANCO 2 antibody
    • Ankyrin repeat domain-containing protein 12 antibody
    • Ankyrin repeat-containing cofactor 2 antibody
    • FLJ20053 antibody
    • GAC 1 antibody
    • GAC 1 protein antibody
    • HGNC:29135 antibody
    • KIAA0874 antibody
    see all

Anti-ANKRD12 antibody 图像

  • Anti-ANKRD12 antibody (ab66309) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 236 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-ANKRD12 antibody (ab66309)参考文献

ab66309 has not yet been referenced specifically in any publications.

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