概述

  • 产品名称Anti-ANKLE2抗体
    参阅全部 ANKLE2 一抗
  • 描述
    兔多克隆抗体to ANKLE2
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cat
  • 免疫原

    Synthetic peptide corresponding to a region within the middle amino acids 864-913 (

    CYSPSDRQSWPSPAVKGRFKSQLPDLSGPHSYSPGRNSVAGSNPAKPGLG

    ) of Human ANKLE2 (NP_055929)

  • 阳性对照
    • 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex.
  • 序列相似性Belongs to the ANKLE2 family.
    Contains 1 ANK repeat.
    Contains 1 LEM domain.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANKL2_HUMAN antibody
    • ANKLE 2 antibody
    • ANKLE2 antibody
    • ankyrin repeat and LEM domain containing 2 antibody
    • Ankyrin repeat and LEM domain-containing protein 2 antibody
    • FLJ 22280 antibody
    • FLJ 36132 antibody
    • FLJ22280 antibody
    • FLJ36132 antibody
    • KIAA 0692 antibody
    • KIAA0692 antibody
    • LEM domain containing 7 antibody
    • LEM domain-containing protein 4 antibody
    • LEMD 7 antibody
    • LEMD7 antibody
    see all

Anti-ANKLE2 antibody 图像

  • Anti-ANKLE2 antibody (ab83830) at 1 µg/ml (in 5% skim milk / PBS buffer) + 10ug 293T cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 104 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)

Anti-ANKLE2 antibody (ab83830)参考文献

ab83830 has not yet been referenced specifically in any publications.

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