Anti-ANKIB1抗体(ab99346)
Key features and details
- Rabbit polyclonal to ANKIB1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ANKIB1抗体 -
描述
兔多克隆抗体to ANKIB1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant -
免疫原
Synthetic peptide within Human ANKIB1 aa 1000-1100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_061877.1
Database link: Q9P2G1 -
阳性对照
- HeLa or 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99346于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 122 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. -
序列相似性
Belongs to the RBR family.
Contains 2 ANK repeats.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 UIM (ubiquitin-interacting motif) repeat. - Information by UniProt
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数据库链接
- Entrez Gene: 54467 Human
- SwissProt: Q9P2G1 Human
- Unigene: 83293 Human
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别名
- AKIB1_HUMAN antibody
- Ankib1 antibody
- ankyrin repeat and IBR domain containing 1 antibody
see all
图片
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All lanes : Anti-ANKIB1 antibody (ab99346) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 30 seconds -
Lane 1: 3µg ab99346 were used to precipitate ANKIB1 from 3mg HeLa whole cell lysate. 20% IP was loaded on gel and probed with ab99346 at 1µg/ml.
Lane 2: same, except IP was carried with a control IgG.
Detection: chemiluminescence with exposure times of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab99346 尚未被引用在任何文献中。