概述

  • 产品名称
  • 描述
    兔多克隆抗体to ANKIB1
  • 经测试应用
    适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 925 and 975 of Human ANKIB11 (NP_061877.1).

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab99345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
    • 序列相似性
      Belongs to the RBR family.
      Contains 2 ANK repeats.
      Contains 1 IBR-type zinc finger.
      Contains 2 RING-type zinc fingers.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AKIB1_HUMAN antibody
      • Ankib1 antibody
      • ankyrin repeat and IBR domain containing 1 antibody
      • Ankyrin repeat and IBR domain-containing protein 1 antibody
      • FLJ33123 antibody
      see all

    图片

    • 3µg ab99345 were used to immunoprecipitate ANKIB1 from 1 mg HeLa whole cell lysate.
      20% IP was loaded on gel and probed with a different anti-ANKIB1 antibody.
      Detection: chemiluminescence with an exposure time of 3 seconds.

    文献

    ab99345 has not yet been referenced specifically in any publications.

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