Anti-ANKHD1抗体(ab117788)
Key features and details
- Rabbit polyclonal to ANKHD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-ANKHD1抗体
参阅全部 ANKHD1 一抗 -
描述
兔多克隆抗体to ANKHD1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 25-75 of Human ANKHD1 (NP_060217.1).
-
阳性对照
- HeLa and 293T whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab117788 was affinity purified using an epitope specific to ANKHD1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117788于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 269 kDa.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
说明 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 269 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
-
功能
May play a role as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. Isoform 2 may possess an antiapoptotic effect and protect cells during normal cell survival through its regulation of caspases. -
组织特异性
Ubiquitous with high expression in cervix, spleen and brain. Expressed in hematopoietic cells with increased expression in leukemia cells. Isoform 2 is highly expressed in spleen with almost no expression in muscle and brain. -
序列相似性
Belongs to the mask family.
Contains 25 ANK repeats.
Contains 1 KH domain. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 54882 Human
- Omim: 610500 Human
- SwissProt: Q8IWZ3 Human
- Unigene: 594084 Human
- Unigene: 720987 Human
-
别名
- ANKH1_HUMAN antibody
- ANKHD 1 antibody
- ANKHD1 antibody
see all
图片
-
All lanes : Anti-ANKHD1 antibody (ab117788) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 269 kDa
Exposure time: 30 seconds -
Detection of ANKHD1 by Western Blot of Immunprecipitate.
ab117788 at 1µg/ml staining ANKHD1 in 293T whole cell lysate immunoprecipitated using ab117788 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab117788 被引用在 2 文献中.
- Lv HW et al. SMYD3 confers cisplatin chemoresistance of NSCLC cells in an ANKHD1-dependent manner. Transl Oncol 14:101075 (2021). PubMed: 33773404
- Liu XF et al. ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway. Int J Oncol 56:1175-1185 (2020). PubMed: 32319569