antigen sequence: GLTMGQQYFV QVSAYNMKGW GPAQTTTPAC ASPSNWKDYD DREPRHKGQS EVLEGLLQQV RALHQHYSCR ESTKLQTTGR KQSVSRSLKH LF, corresponding to internal sequence amino acids 336-427 of Human ANKFN1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 2 ANK repeats.
Contains 1 fibronectin type-III domain.
Information by UniProt
- ANKF1_HUMAN antibody
- ANKFN1 antibody
- Ankyrin repeat and fibronectin type-III domain-containing protein 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ANKFN1 antibody (ab121684)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121684 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKFN1 antibody (ab121684)
ab121684 at 1/10 dilution staining ANKFN1 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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