• 产品名称Anti-Anillin抗体
    参阅全部 Anillin 一抗
  • 描述
    兔多克隆抗体to Anillin
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to a region within residue 1074 - 1124 of Human Anillin (NP_061155.2)

  • 阳性对照
    • Colon carcinoma; breast carcinoma.



Our Abpromise guarantee covers the use of ab84676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may enhance staining.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
    • 组织特异性Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
    • 序列相似性Contains 1 PH domain.
    • 发展阶段Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
    • 翻译后修饰Phosphorylated during mitosis.
      Ubiquitinated, and this requires FZR1/CDH1.
    • 细胞定位Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Actin binding protein scraps homolog Drosophila antibody
      • Actin binding protein anillin antibody
      • Actin-binding protein anillin antibody
      • Anillin actin binding protein antibody
      • anln antibody
      • ANLN_HUMAN antibody
      • DKFZp779A055 antibody
      • Scra antibody
      • Scraps antibody
      • Scraps homolog antibody
      see all

    Anti-Anillin antibody 图像

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma using 1/250 ab84676, followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma using 1/250 ab84676, followed by DAB staining.

    Anti-Anillin antibody (ab84676)参考文献

    ab84676 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (colon)
    Specification colon
    Fixative Acetone
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buufer
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

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    提交于 Dec 22 2012